MEET THE OT-2
Lab automation has never been easier
The high-precision OT-2 lab robot is flexible, open-source, and user-friendly.
Ideal for:
- Genomics
- Proteomics
- Cell-based assays
- Biochemical assays
- Drug discovery
flexible hardware
One robot, countless workflows
- Configurable 11-deck platform with options for Magnetic, Temperature and Thermocycler Modules
- Swappable single & 8-channel pipettes for multiple volume configurations
- Standardized (SBS) deck slots allow you to use labware you already own
Performance
- Accuracy: 15% at 1uL
- Precision: 5% at 1uL
- Throughput: Fast fill a 96-well plate in 22 seconds
flexible software
Powerful software at your fingertips
- Choose from free, beginner-friendly & advanced protocol controls
- Powerful cloud-based tools
- Unmatched control of nearly every aspect of your workflow
User Friendly
- Self-serve unboxing and installation
- Reagent and bead system agnostic
- Plug-and-play setup with access to protocol libraries to enable immediate assay implementation
- Easily integrates to your existing instruments
- Compatible with ANSI/SBS compliant or automation friendly tubes, microplates, deep-well plates, reservoirs and adapters
open source
Near limitless control
- 100% customizable and open to modifications adjustable to your assay
- Modify or customize protocols with our simple yet powerful Python API
- Digital protocol commands surpass manual pipetting capabilities
Updates
- Supports Ethernet or USB connection (external dongle provided)
- 4 USB ports available to support connection to multiple modules
- Easy access to electronics via external PCBA enclosure
- LED indicator provide users feedback, diagnosis & troubleshooting aid on instrument power state, system processing, ethernet, and network connectivity
Beginner-Friendly
So easy, you’ll feel like an automation expert
Real-time chat support on your computer or mobile phone.
Plug-and-play. Unbox, set-up, calibrate. No technician needed.
Dedicated Account Managers available to you at every step.
Start with confidence. Demo the OT-2 for 60 days.
Specs
Lab automation rethought from the ground up
- Compact footprint
- 63cm x 57cm x 66cm / 25in x 22.5in x 26in (W,D,H)
- Takes up half of your standard lab bench
- Pipette configurations
- Single and 8-channel pipetting
- 2-pipette mounts, for a configuration of 1 or 2 single or 8-channel pipettes. Pipettes are easily interchangeable.
- Pipette volumes and specs
- Single channel: 1-1000µl
- 8-channel: 1-300µl
- Opentrons Pipette Whitepaper (GEN1 & GEN2) ›
- Single channel manual ›
- 8-channel manual ›
- Deck
- 11 deck slots enable countless configurations
- Deck slots are compatible with standard SBS dimensions. Deck also includes a removable trash bin.
- Connectivity
- WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0
- Learn more ›
- Weight
- 48kg or 105.8 lb
- Video
- Integrated video camera (Included for future or custom functionality)
- Audio
- Integrated audio system (Included for future or custom functionality)
- Power Requirements
- Robot Power Input: 36VDC, 6.1A
- Power Adapter Input: 100-240VAC, 50/60Hz, 4.0A/115VAC, 2.0A/230VAC
- For additional power requirement information, please review this support article
- Frame composition
- Rigid steel and CNC aluminum design
- Operating Temperature & Relative Humidity
- Recommended 20-24C Recommended 40-60% RH
- Learn more ›
Speed
Fills a 96-well plate in 22 seconds
| Pipette | Distribute, One Tip | Transfer, Same Tip | Transfer, New Tip |
|---|---|---|---|
P20 8-channel | 0:22 | 1:35 | 4:19 |
P300 8-channel | 0:26 | 1:38 | 4:24 |
P20 Single | 3:12 | 13:10 | 36:01 |
P300 Single | 4:01 | 13:39 | 41:31 |
P1000 Single | 5:54 | 15:21 | 44:50 |
Accuracy and precision
Single and 8-channel pipetting
| Pipette | Volume | Random Error | Systematic Error | ||
|---|---|---|---|---|---|
| %D | µl | %CV | µl | ||
| p20 | 1 | ±15% | 0.15µL | ±5% | 0.05µL |
| 10 | ±2% | 0.2µL | ±1% | 0.1µL | |
| 20 | ±1.5% | 0.3µL | ±0.8% | 0.16µL | |
| p300 | 20 | ±4% | 0.8µL | ±2.5% | 0.5µL |
| 150 | ±1% | 1.5µL | ±0.4% | 0.6µL | |
| 300 | ±0.6% | 1.8µL | ±0.3% | 0.9µL | |
| p1000 | 100 | ±2% | 2.0µL | ±1% | 1µL |
| 500 | ±1% | 5.0µL | ±0.2% | 1µL | |
| 1000 | ±0.7% | 7.0µL | ±0.15% | 1.5µL | |
| Pipette | Volume | Random Error | Systematic Error | ||
|---|---|---|---|---|---|
| %D | µl | %CV | µl | ||
| P20 | 1 | ±20% | 0.2µL | ±10% | 0.1µL |
| 10 | ±3% | 0.3µL | ±2% | 0.2µL | |
| 20 | ± 2.2% | 0.44µL | ±1.5% | 0.3µL | |
| P300 | 20 | ±10% | 2.0µL | ±4% | 0.8µL |
| 150 | ±2.5% | 3.75µL | ±0.8% | 1.2µL | |
| 300 | ±1.5% | 4.5µL | ±0.5% | 1.5µL | |
Sterility
Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment
Add the Opentrons HEPA Module, or add your own UV lights
Viscosity
For best practices on automating viscous liquid handling, review our application note
- API compatibility
- Interfaces with any product that has an API
- Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.
- Learn more ›
- Interface
- Windows 10, macOS 10.10 or later, Ubuntu 12.04 or later.
- Opentrons App ›
- Tip compatibility
- Use Opentrons Tips and Opentrons Filter Tips
- Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.
- Labware compatibility
- Use common labware already in our Labware Library, or add your own
- If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.
- Learn more ›
- OT-2 Manual ›
- Warranty
- One year warranty against manufacturer defects
- Stress tested to last a minimum of 3 years of full-time use
- Learn more ›
- Return Policy
- 60 day, 100% risk free
- Learn more ›
- Certifications
- Certifications complete (CE, FCC)
- Certifications pending (NRTL, CB)
- Not certified/validated (IVD, GMP, ISO)
Tip Handling
- Pick up tip
- Drop tip
- Return tip
- New tip (always, never, once)
- More...
Liquid Control
- Aspirate
- Dispense
- Aspirate speed
- Dispense speed
- Blowout
- Touch tip
- Mix
- Air gap
Utility
- Move to
- Delay
- User-specified pause
- Homing
- Comment
Transfer
- Basic transfer
- Volumes > Pipette Volume
- Distribute
- Consolidate
Variable Settings
- Speed
- Volume
- Count
Other
- Dispose
Welcome To Opentrons!
How The Opentrons Platform Works
Custom Modular Hardware
Setting Up Your OT-2
OT-2 Hardware Video
Risk free 60-day demo
Try the OT-2 in your lab today and take advantage of protocols designed for sharing and editing.
